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Biophysical modeling leads to newly discovered molecular interactions between the ribosome and mRNA at standby sites, and predicts how sequences upstream of the Shine-Dalgarno control translation rate and post-transcriptional regulation.

Espah Borujeni, A., Channarasappa A.S., and Salis H.M., (2013) Translation Rate is Controlled by Coupled Trade-offs between Site Accessibility, Selective RNA unfolding and Sliding at Upstream Standby Sites, Nucleic Acid Research, v41 (21)

Learn the origins of "context effects" between promoters and RBSs, and how structured mRNAs can coax the ribosome into performing regulatory tricks.

RBS Library Calculator
Title


Pre-Sequence [?]
Pre-Sequence: enter a nucleotide sequence (5 to 20 bp) that appears before the ribosome binding site, using A/G/C/T/U. The Pre-Sequence is important when the ribosome binding site is less than 35 nucleotides long. (optional)
Protein Coding Sequence [?]
Protein Coding Sequence: enter a nucleotide sequence (at least 50 bp) that encodes a protein, using A/G/C/T/U. Begins with a start codon (ATG/GTG/TTG/CTG). (required)
 


Initial RBS Sequence with Optional Constraints [?]
Degenerate Ribosome Binding Site Sequence: enter a degenerate nucleotide sequence that defines a library of RBSs, using the 15 letter UIPAC degenerate nucleotide code: A/G/C/T/S/W/K/R/Y/M/D/B/H/V/N. S: G or C. W: A or T. K: G or T. R: A or G. Y: C or T. M: A or C. D: A, G, or T. B: C, G, or T. H: A, C, or T. V: A, C, or G. N: A, G, C, or T.
(optional)

Minimum Translation Initiation Rate [?]
RBS Library's Minimum Translation Initiation Rate: select the desired minimum rate of translation initiation within the optimized ribosome binding site library, on a proportional scale from 0.1 to 100,000 or more. This will determine the lowest protein expression level within the library. (required)
Maximum Translation Initiation Rate [?]
RBS Library's Maximum Translation Initiation Rate: select the desired maximum rate of translation initiation within the optimized ribosome binding site library, on a proportional scale from 0.1 to 100,000 or more. This will determine the highest protein expression level within the library. (required)


Estimated Library: x     Low Res    
    High Res
Organism or (16S rRNA) [?]
Organism or 16S rRNA sequence: choose a bacterial species by typing in the first 3 letters of its name and selecting it from the list. Alternatively, you may enter the last 9 nucleotides of the 16S rRNA, using A/G/C/T/U. (required)
(start typing)

For Non-Commercial Use Only
Design Jobs: 1 queued, 48 currently running
When using these results, please reference: Iman Farasat, Jason Collens, Matthew Guido, and Howard Salis, "Efficient search for optimal expression levels in multi-protein genetic systems", in review
Method online since January 10th, 2011.
Have a Question? Our Documentation, Publications, and References may have your answer!
We gratefully acknowledge research funding from the Defense Advanced Research Projects Agency, the National Science Foundation, the Office of Naval Research, and an Amazon AWS Research Grant.
Computational resources are provided by the AWS Elastic Compute Cloud.